Efficient genetic transformation of Momordica charantia L. by microprojectile bombardment

Transformation of Filamentous Fungi by Microprojectile Bombardment

The vast majority of transformation protocols for filamentous fungi are based on permeabilizing cell membranes with polyethylene glycol (PEG) or electroporation. Both methods typically require preparing protoplasts or osmotically sensitive cells prior to transformation (Herzog et al., 1996; Goldman et al., 1990; a detailed review of fungal transformation systems is given by Lemke and Peng, 1995...

Genetic Transformation of Immature Sorghum Inflorescence via Microprojectile Bombardment

Transformation of Aspergillus nidulans by microprojectile bombardment on intact conidia.

This paper describes transformation of intact conidia of Aspergillus nidulans, auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/microgram of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integrat...

Genetic Divergence Studies in Bitter Gourd (Momordica charantia L.)

A study was conducted during Zaid 2005, in Uttar Pradesh, India, to evaluate the nature and magnitude of genetic divergence in 30 bitter gourd genotypes. Results revealed the presence of wide genetic diversity. The genotypes were grouped into 6 clusters based on Mahalanobis D statistics using Tocher's 2 method. The clustering pattern of genotypes revealed that the genetic diversity was independ...

Inheritance of gynoecism in bitter gourd (Momordica charantia L.).

The inheritance of sex expression in cucumber (Cucumis sativus) and other cucurbits is well documented; however, the genetics of female sex (gynoecism) expression in bitter gourd (Momordica charantia) has not been described. Inheritance of gynoecism in bitter gourd was studied in a 100% gynoecious line (Gy263B). The F(2) and testcross segregation data revealed that gynoecism in Gy263B is under ...